轴突中含有轴向细胞骨架，负责将合成于神经元胞体内、含有神经递质的囊泡运输到轴突末端的突触前膜。
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为此，研究团队使用高压冷冻技术来处理不同的小鼠神经元样本，包括在实验室培养皿中生长的神经元、从成年小鼠以及小鼠胚胎中提取的神经元切片。研究作者指出，这项技术可以更好地保存细胞的精细结构。需要指出的是，这些神经元轴突没有最外层的髓鞘包裹，由此避免了髓鞘对轴突形态的限制。
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